The effects of domestic recombinant adenovirus-P53(rAd-P53) with DDP on growth and apoptosis of ovarian carcinoma cell lines
[Abstract] Objective: To evaluate the effects of domestic recombinant adenovirus-P53 (rAd-P53,Gendicine) and rAd-P53 with DDP on growth and apoptosis of ovarian carcinoma cell lines. Methods: using MTT、flow cytometry、Western blot to compare ovarian carcinoma cell lines growth inhibition rate、cell cycle and apoptosis of ovarian carcinoma cell lines and to detect P53 expression of ovarian carcinoma cell lines after treated with rAd-P53 72 hours later. Results: 1. There was a dose-dependent and time-dependent inhibition of cell proliferation by rAd-P53. After combined treatment, the growth inhibition rate was significant higher(P<0.01). The sequence of combined treatment is of no difference to the cell growth inhibition(P>0.6). 2. Combined administration significantly arrested ovarian carcinoma cell lines in G0/G1, S phase significantly decreased and the apototic rate was significantly higher(P<0.01). 3. High level P53 expression was dectected in ovarian carcinoma cell lines by Western blot after administeration of rAd-P53 72 hours later. Conclusion: rAd-P53 can tranfect wide-type P53 into the genome group of human ovarian tumor cells, arrest tumor cells at G0/G1, inhibit the growth of tumor cells, promote tumor cells apoptosis. Its combination with DDP may significantly enhance DDP antitumor effect.
[Key words] ovarian neoplasms; gene therapy; P53; cisplatin
